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Clinical microbiology laboratory plays several important roles in the management of bacterial infections.Isolation, identification of pathogenic microorganisms in cultures and subsequent antimicrobial susceptibility testing always assists in selecting appropriate antimicrobial agent and prevention of unnecessary complications.This method involves measuring the inhibition of bacteria growth around an antibiotic disc placed in a culture. How did each antibiotic perform in each bacteria culture? How could you continue this study to discover even more about the relationship of antibiotics and bacteria?
After you have tried that, stain each of your live bacteria cultures using the following procedure: Once you have determined which of your live cultures is gram-negative and which is gram-positive, label them clearly and move on to the next part of the experiment.
One way to test bacteria susceptibility to antibiotics is to use the Kirby-Bauer or ‘disc diffusion’ method. What do your results tell you about the process of prescribing antibiotics?
Gram staining helps doctors make a diagnosis, but can it also help suggest a cure?
What is the relationship between gram classification and antibiotic use?
Always carefully read the entire label before using a chemical.
Be sure you understand the hazards involved, the proper safety equipment to wear, and what you will do in case of a spill or contact with your skin.
When you have finished studying a culture, pour enough household bleach into it to cover the bottom of the dish.
Then cover the culture, seal it in a plastic bag, and throw it away.
Work in a clean, well ventilated, uncluttered area where you can quickly wipe up spills. While most environmental bacteria are not harmful to healthy individuals, once concentrated in colonies, they can be hazardous.
To minimize risk, wear disposable gloves while handling bacteria, and thoroughly wash your hands before and after.